Welcome to Sensimix Real-time PCR Kits

Sensimix Real-time PCR Kits

Bioline's SensiMix Kits are a complete range of highly optimized ready-to-use kits, designed to deliver outstanding results for real-time PCR experiments with both DNA and RNA templates. Powered by a highly-sensitive hot-start DNA polymerase, SensiMix formulations facilitate very rapid enzyme-extension rates, enabling fast cycling conditions to be used without compromising PCR efficiency, target detection sensitivity, specificity or reproducibility. SensiMix Kits give reliable and highly reproducible data on all real-time PCR instruments, thereby reducing the optimization required for even the most challenging assays.

DNA SYBR Kits


SensiMix SYBR kits contain all the components necessary to perform consistent and reproducible real-time PCR assays with your DNA template. The 2x mastermixes deliver highly specific and sensitive results across a wide dynamic range of template concentrations (over 10 fold), producing reliable real-time data quickly and easily. The kits are suitable for DNA templates or RNA templates following reverse transcription with the Bioline

Description :

SensiMix SYBR Kit
Cat No. Size Conc.
QT605-02 2x 250 Reactions
QT605-05 2x 500 Reactions
QT605-20 2x 2000 Reactions

Features and Benefits

* Simple and reproducible: a choice of optimized kits for different platforms, just add primers and template, reducing possible errors in set-up
* Specific and sensitive: for detection of a wide range of template concentration, even from hard to obtain and low copy number samples
* Fast protocols: can be used with the new generation of fast cyclers without compromising performance
* Flexible: validated with all major real-time instruments
* 2x master mix: premixed with SYBR Green I and an internal reference for convenience

Instrument Compatibility

ABI 7000, 7300, 7700, 7900, 7900HT and StepOne™. (See product selection table).
However the kit is also compatible with several instruments that do not require the use of ROX™, such as the Qiagen Rotor-Gene™ 6000, the Bio-Rad CFX96 or the Roche LightCycler® 480.

Description

Versatility

* Mix optimised for SYBR® Green I chemistry.
* Suitable for high throughput applications e.g. target validation for drug discovery.
* Designed to work across a range of block and rotor-based instruments.

SensiMix SYBR kit contains all the components necessary to perform consistent and reproducible real-time PCR assays with your DNA template. The kits deliver highly specific and sensitive results across a wide dynamic range of template concentrations (over 10 fold), producing reliable real-time data quickly and easily. The kits are suitable for DNA templates or RNA templates following reverse transcription with the Bioline cDNA Synthesis Kit (BIO-65025) and are compatible with the ABI 7000, 7700, 7900HT and StepOne™ platforms as well as instruments that do not require the use of ROX. (See product selection table).

The kits are powered by a highly-sensitive hot-start DNA polymerase that provides higher specificity and ROX, making it specifically suitable for use on real-time instruments that measure this ROX signal.

Volume

QT605-02: 250 x 50µl Reactions: 5 x 1.25ml
QT605-05: 500 x 50µl Reactions: 10 x 1.25ml
QT605-20: 2000 x 50µl Reactions: 40 x 1.25ml

Components

2x Reaction mix consisting of:

* Heat-activated DNA polymerase
* Ultra-pure dNTPs
* MgCl2 (6mM)
* Internal reference
* SYBR® Green I

Kit Size

The kit size is based on a 50µl final reaction volume.

Storage Conditions

6 months at -20°C. Avoid exposure of the SYBR® Green I to light.

Product Citations

1. Lappas M., et al. Placenta 30(3): 256-262 (2009)
2. Rosker C., et al. J. Biol. Chem. 284(8): 5186-5194 (2009)
3. Barker H.E, et al. BMC Developmental Biology 8(1): 94 (2008)
4. Johnson C.E. et al. StemCells 25: 1913–1923 (2007)