Welcome to Sensimix Real-time PCR Kits

Sensimix Real-time PCR Kits

Bioline's SensiMix Kits are a complete range of highly optimized ready-to-use kits, designed to deliver outstanding results for real-time PCR experiments with both DNA and RNA templates. Powered by a highly-sensitive hot-start DNA polymerase, SensiMix formulations facilitate very rapid enzyme-extension rates, enabling fast cycling conditions to be used without compromising PCR efficiency, target detection sensitivity, specificity or reproducibility. SensiMix Kits give reliable and highly reproducible data on all real-time PCR instruments, thereby reducing the optimization required for even the most challenging assays.

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The SensiMix Probe One Step Kit contains a ready-to-use reagent for probe-based real-time RT-PCR for use on all real-time instruments. The kit has been formulated for use with probe-detection technology, including TaqMan®, Scorpions® and molecular beacon probes. The SensiMix Probe One-Step Kit provides high-performance, reproducible first strand cDNA synthesis and subsequent real-time PCR assays, using a single protocol. Both cDNA synthesis and PCR are performed in a single tube using gene-specific primers and either total RNA or mRNA. The kit combines a reverse transcriptase and a hot-start DNA polymerase, for high specificity and sensitivity of the one-step RT-PCR. SensiMix is inactivate and possesses no polymerase activity during the reaction set-up preventing non-specific amplification including primer-dimer formation.

Graph description :

SensiMix Probe One-Step Kit
Cat No. Size Conc.
QT725-02 2x 250 Reactions
QT725-05 2x 500 Reactions
QT725-20 2x 2000 Reactions
QT725-01 2x 25 Reactions

Features and Benefits

* Reproducible: master-mix contains reverse transcriptase for efficient cDNA synthesis together with an optimized amount of highly engineered hot-start polymerase, to provide reproducible real-time data
* Sensitive: accurate quantification of any RNA from low copy targets. (i.e., pathogen detections)
* Convenient: reduced pipetting steps and possible contamination and RNA degradation

Instrument Compatibility

ABI 7000, 7300, 7500, 7700, 7900, 7900HT, StepOneTM, StepOneTM Plus, Roche LightCycler® 480, Bio-Rad iCycler®, iQTM5 and MyiQTM, OpticonTM, OpticonTM2, Chromo4TM, MiniOpticonTM, CFX96TM, CFX384TM, Cepheid SmartCycler®, Corbett Rotor-GeneTM 3000, Rotor-GeneTM 6000, Eppendorf Mastercycler® ep realplex, Techne Quantica®, Stratagene Mx4000®, Mx3000P® and Mx3005P®. (See product selection table).

Description

The SensiMix Probe One-Step Kit provides optimal performance and maximum convenience for real-time, quantitative analysis of RNA templates in a single reaction format on real-time instruments such as the Corbett Research Rotor-Gene™ 3000 and Roche LightCycler® 480. A separate tube of ROX fluorescent dye is supplied as an option to measure this signal on real-time instruments such as the ABI 7500, 7900HT and the Stratagene Mx3000P® and Mx3005P® platforms. (See product selection table).

The SensiMix Probe One-Step Kit has been optimized for use on a wide variety of probe chemistries including TaqMan® probes, molecular beacons, scorpions and FRET probes. Applications include real-time PCR and allelic discriminations

Volume

QT725-01: 25 x 50µl Reactions: 1 x 625µl
QT725-02: 250 x 50µl Reactions: 5 x 1.25ml
QT725-05: 500 x 50µl Reactions: 10 x 1.25ml
QT725-20: 2000 x 50µl Reactions: 40 x 1.25ml

Components

2x Reaction mix consisting of:

* Heat-activated DNA polymerase
* Sensi reverse transcriptase
* Internal reference
* Ultra-pure dNTPs
* MgCl2 (6mM)

Plus separate vials of:

* 50mM MgCl2
* RNase inhibitor
* DEPC-treated water
* ROX Internal reference 25µM

 

Kit Size

The kit size is based on a 50µl final reaction volume.

Storage Conditions

6 months at -20°C. Avoid exposure of the SYBR® Green I to light.